Which method is known for DNA sequencing?

The Sanger method is recognized as one of the fundamental techniques for DNA sequencing developed by Frederick Sanger in the 1970s. This method relies on the incorporation of chain-terminating dideoxynucleotides during DNA replication. When these modified nucleotides are incorporated into the growing DNA strand, they prevent further elongation, effectively "terminating" the synthesis at specific points. By performing this process in a controlled manner, one can generate fragments of various lengths corresponding to the sequence of the template DNA.

These fragments are then separated by size using techniques such as electrophoresis, allowing researchers to read the sequence of nucleotides from the shortest to the longest fragment. The specific sequence of bases can then be determined by analyzing the order in which the dideoxynucleotides were incorporated. The Sanger method was the basis for the first generation of DNA sequencing technologies and is still widely used today in various applications, including the sequencing of small to medium-sized DNA samples.